Coding

Part:BBa_K4260011:Design

Designed by: Claudia Angélica García Alonso   Group: iGEM22_TecCEM   (2022-10-09)


Coding sequence for Isoeugenol Monooxygenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 66
    Illegal NgoMIV site found at 774
    Illegal NgoMIV site found at 1382
    Illegal AgeI site found at 598
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 694


Design Notes

Codon optimization was performed for both coding sequences.

Source

The Isoeugenol monooxygenase comes from Pseudomonas putida IE27.

References